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Dernière mise à jour : Mai 2018

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1st DNAqua-Net International Conference : l\'ADN environnemental pour la biosurveillance des milieux aquatiques.

DNAqua-Net organizers

DNAqua-Net Management Team 

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Florian LEESE,

Germany

Agnès BOUCHEZ,

France

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Alexander WEIGAND,
Luxembourg

Sarah KUCKMANN,
Germany

Charlotte FRIE,
Germany

 “New DNA and eDNA-based tools emerged rapidly over the past years. Undoubtedly, they can significantly complement and potentially replace some of the current bioassessment and -monitoring approaches. However, while innovative and powerful, it is difficult to identify 'THE' optimal way of DNA and eDNA-based assessments and especially end-users are puzzled by the sheer number of options and protocols. The main aim of DNAqua-Net is thus to connect researchers, service providers and end-users to identify consensus and derive best practice strategies, ideally standards.   While based in Europe, DNAqua-Net’s scope goes far beyond Europe and partners from 49 countries participate in the network."

 WG1 - DNA Barcode References

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Fedor ČIAMPOR,
Slovakia

Torbjorn EKREM,
Norway

 “The main task of WG1 was to identify gaps for aquatic species in ongoing national DNA barcoding projects and to assess the suitability of DNA barcode reference databases for regulatory biomonitoring. Our team collected data from all over Europe and provided very detailed answers. In addition, during the workshop, we curated databases and filled important gaps. The main result of our analyzes is that future work must focus on completing and repairing a reference database that will provide a backbone for taxon-based aquatic biomonitoring.”

WG2 - Biotic Indices & Metrics

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Maria KAHLERT,

Sweden

Jan PAWLOWSKI,
Switzerland, Poland

 "The aim of WG2 was to assess the ecological value of new bioindicators based on environmental DNA barcoding and discuss the revision of currently used biotic indices to eventually adapt them the specificity of DNA data. We studied the difference of data and indices derived from DNA and eDNA-based methods compared to classical morphotaxonomic assessments in the context of ongoing monitoring programs. Specifically, we asked if novel genetic methods and DNA-based data types are compatible for bioassessment and monitoring. We found that while current indices and methods can be adapted to DNA methods, novel and powerful ecogenomic indicators can be derived as well. These novel indices have the potential to inform reliably about ecological status using so far unconsidered indicator taxa, and their value needs further testing."

 WG3 - Field & Lab Protocols

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Kat BRUCE,
United Kingdom

Emre KESKIN,
Turkey

 "DNA-based methods for bioassessment emerge all around the globe. Most labs have individual preferences for field and lab processing. Through many research exchanges and trainings, WG3 has tested and evaluated different approaches and provided guidance on critical steps in the process. A main outcome of WG3 are guidance documents as well as minimum criteria to be considered in formal standardization of field and lab protocols."

 WG4 - Data Analysis & Storage

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Diego FONTANETO,

Italy

Alain FRANC,

France

“Similar to lab and field protocols there is a huge diversity of bioinformatic processing approaches when it comes to the analysis of DNA metabarcoding data. In our working group we compared how decisions on certain settings impact on the resulting OTU and taxon tables. In addition, we developed approaches for how analyses can build on available large scale research infrastructure on massive NGS data in order to pave the way towards a more harmonized analysis of DNA metabarcoding data.”

 WG5 - Implementation Strategy & Legal Issues  

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Patricia MERGEN,
Belgium

Daniel HERING,
Germany

Pieter BOETS,
Belgium

“Undoubtedly, DNA-based methods can support environmental management. However, the way towards the inclusion of novel genetic tools into ongoing regulatory biomonitoring programs is anything but trivial. In WG5 we liaised with partners from all around the globe to discuss roadmaps towards implementation in different contexts (e.g., Water Framework Directive, Marine Strategy Framework Directive). In particular we addressed where it is already possible to develop standards in order to consolidate the process and facilitate implementation. The main challenge is to get the method implemented in regular biomonitoring and get it accepted at EU level to be used next to traditional biomonitoring methods.”