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INRA
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Dernière mise à jour : Mai 2018

Menu Logo Principal logo aquanet logo inrae logo leeselab logo COST

1st DNAqua-Net International Conference : l\'ADN environnemental pour la biosurveillance des milieux aquatiques.

Schedule

The presentations whose authors wish will be available later on a YouTube channel.

Program at a glance 

dnaqua-planning-V7

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Detailed Schedule

Schedule download (Excel File)  

  Day1     Day2      Day3

 

March 9 PM

13:00 - 13:05  Agnès Bouchez
Welcome address

13:05 - 13:15  Florian Leese, Agnès Bouchez
DNAqua-Net achievements and perspectives 

13:15 - 13:40  Kristy Deiner (Keynote Lecture)
Authors: Kristy Deiner
The challenges we've overcome and the new opportunities for using environmental DNA in biomonitoring aquatic resources

13:40 - 14:05  Hideyuki Doi (Keynote Lecture)
Authors: Hideyuki Doi
Environmental DNA methods for the analysis of macroorganismal populations

DNA Barcode References - WG1 session
Chairs: Torbjørn Ekrem, Fedor Čiampor Jr

14:05 - 14:15  Torbjørn Ekrem, Fedor Čiampor Jr (Session Introduction)
Authors: Torbjørn Ekrem, Fedor Čiampor Jr
DNA barcode reference libraries: Achievements of the DNAqua-Net Working Group 1

14:15 - 14:40  Hannah Weigand (Keynote Lecture)
Authors: Hannah Weigand
Mind the gap-analysis! – How complete are DNA barcode reference libraries for monitoring-relevant aquatic species in Europe?

14:40 - 15:05  Filipe Costa (Keynote Lecture)
Authors: Filipe Costa
Venturing into auditing of reference libraries: from the hackathon on marine invertebrates to sorting with BAGS

15:05 - 15:15  10-min break

15:15 - 15:30  Magdalini Christodoulou
Authors: Magdalini Christodoulou, Berry van der Hoorn, Laure Van den Bulcke, Sofie Derycke, Annelies De Backer, Ingrid Kröncke, Pedro Martinez Arbizu
A reliable DNA barcode reference library for the identification of benthic invertebrates: essential for biomonitoring of the North Sea

15:30 - 15:45  Cristina Claver
Authors: Cristina Claver, Naiara Rodriguez-Ezpeleta, Oriol Canals
Assessing accuracy and completeness of GenBank for eDNA metabarcoding: towards a reliable marine fish reference database

15:45 - 15:50  Anna Wysocka
Authors: Anna Wysocka, Michal Grabowski, Lidia Sworobowicz, Sasho Trajanovski, Tomasz Mamos
DNA barcoding in recognition of Gammarus flock diversity and distribution in the ancient Lake Ohrid

15:50 - 16:00  10-min break

16:00 - 16:15  Virginie Marques
Authors: Virginie Marques, Tristan Milhau, Camille Albouy, Tony Dejean, Stéphanie Manel, David Mouillot, Jean-Baptiste Juhel
GAPeDNA: Assessing and mapping global species gaps in genetic databases for eDNA metabarcoding

16:15 - 16:30  Laura Gargan
Authors: Haris Zafeiropoulos, Laura Gargan, Christina Pavloudi, Evangelos Pafilis, Jens Carlsson
Bacteria are everywhere, even in your COI data: Τhe art of getting to know the unknown unknowns and shine light on the dark matter!

16:30 - 16:45  Francesco Zangaro 
Authors: Maurizio Pinna, Francesco Zangaro, Eftychia Tzafesta, Valeria Specchia
How many non-indigenous species occurring in the Mediterranean Sea can actually be identified through environmental DNA? A gap analysis for NIS DNA barcode in the reference libraries

16:45 - 17:00  Patrik Macko
Authors: Patrik Macko, Tomáš Derka, Fedor Čiampor Jr, Zuzana Čiamporová-Zatovičová
The first step towards a DNA barcode reference database for mayflies (Ephemeroptera) of Slovakia

17:00 - 17:15  Tomasz Mamos
Authors: Tomasz Mamos, Tomasz Rewicz, Karolina Bącela-Spychalska, Aleksandra Jabłońska, Kamil Hupało, Krešimir Žganec, Misel Jelic, Remi Wattier, Anna Wysocka, Lidia Sworobowicz, Denis Copilaş-Ciocianu, Adam Petrusek, Jelena Hinić, Valentina Slavevska Stamenkovic, Michal Grabowski
DNA barcode reference library for European freshwater malacostracans: getting there

17:15 - 17:25  10-min break

17:25 - 17:30  Michal Grabowski
Authors: Remi Wattier, Denis Copilas-Ciocianu, Mišel Jelić, Anthony Ollivier, Arnaud Chaumot, Michael Danger, Vincent Felten, Christophe Piscart, Krešimir Žganec, Tomasz Rewicz, Anna Wysocka, Thierry Rigaud, Michal Grabowski
Continental‑scale patterns of hyper‑cryptic diversity within the freshwater model gammarid taxon

17:30 - 17:35  Raşit Bilgin
Authors: Rasit Bilgin, Kübra Karaman, Yağmur Tarhana, Ceylan Yücel, Elizabeth Hemond, Mehmet Yokeş, Evrim Kalkan, Selahattin Karhan
CO1 Metabarcoding of Marine Water Samples from the 2nd Turkish Antarctic Expedition (TAE2) Highlights the Deficiencies in the Reference Barcoding Databases for the Continent

17:35 - 17:40  Magdalena Gajdošová
Authors: Magdalena Gajdošová, Arne Beermann, Jindřiška Bojková, Vendula Polášková, Jana Schenková, Marie Zhai, Michal Horsák, Florian Leese, Adam Petrusek
Community metabarcoding uncovers vast diversity of aquatic invertebrates in the Carpathian spring fens

17:40 - 17:45  Liz Davidson
Authors: Liz Davidson
DNA barcodes for UK freshwater arthropod species: coverage, quality and implications

17:45 - 17:50  Piotr Gadawski
Authors: Piotr Gadawski, Matteo Montagna, Bruno Rossaro, Wojciech Giłka, Vladimir Pesic, Michal Grabowski, Giulia Magoga
DNA barcoding reveals an unknown Chironomidae diversity from the freshwater biodiversity hot-spot: comparison between local and the European datasets.

17:50 - 17:55  François Keck
Authors: François Keck, Florian Altermatt
From DNA sequences to operational reference databases: an opinionated approach using R

17:55 - 18:00  10-min break

18:00 - 20:00  Poster session WG1 & WG3

March 10 AM

Field & Lab Protocols - WG3 session
Chairs: Kat BRUCE, Emre KESKIN

09:00 - 09:10  Kat Bruce, Emre Keskin (Session Introduction)
Authors: Kat Bruce, Emre Keskin
Field and Lab Protocols: Achievements of the DNAqua-Net Working Group 3

09:10 - 09:35  Xavier Pochon (Keynote Lecture)
Authors: Xavier Pochon, Susie Wood, Javier Atalah, Lauren Fletcher, Olivier Laroche, John Pearman, Deanna Elvines, Anastasija Zaiko, Laura Biessy, Georgia Thomson-Laing, Nigel Keeley
A validated protocol for fish farm monitoring using environmental DNA

09:35 - 10:00  Kristine Bohmann (Keynote Lecture)
Authors: Kristine Bohmann, Christian Carøe
Labelling strategies in metabarcoding studies & how to ensure that nucleotide tags stay in place

10:00 - 10:15  Bettina Thalinger
Authors: Bettina Thalinger, Kristy Deiner, Lynsey Harper, Helen Rees, Rosetta Blackman, Daniela Sint, Michael Traugott, Caren Goldberg, Kat Bruce
An assay validation framework to compare and evaluate targeted environmental DNA assays for routine species monitoring

10:15 - 10:30  Rein Brys
Authors: Rein Brys, Teun Everts, David Halfmaerten, Sabrina Neyrinck, Quentin Mauvisseau
The use of multiple markers and internal positive controls significantly improves species eDNA detection rates and data reliability

10:30 - 10:45  Valentin Vasselon
Authors: Valentin Vasselon, Éva Ács, Salomé Almeida, Karl Andree, Laure Apothéloz-Perret-Gentil, Bonnie Bailet, Ana Baricevic, Kevin Beentjes, Juliane Bettig, Agnès Bouchez, Camilla Capelli, Cécile Chardon, Mónika Duleba, Tina Elersek, Clémence Genthon, Melanie Hurtz, Louis Jacas, Maria Kahlert, Martyn Kelly, Matthieu Lewis, Jan Macher, Federica Mauri, Marina Moletta-Denat, Andreia Mortágua, Jan Pawlowski, Javier Pérez Burillo, Martin Pfannkuchen, Erik Pilgrim, Panayiota Pissaridou, Jonathan Porter, Frederic Rimet, Karmen Stanic, Kálmán Tapolczai, Susanna Theroux, Rosa Trobajo, Berry van der Hoorn, Marlen Vasquez Hadjilyra, Kerry Walsh, David Wanless, Jonathan Warren, Jonas Zimmermann, Maša Zupančič
The Fellowship of the Ring Test : DNAqua-Net WG2 initiative to compare diatom metabarcoding protocols used in routine freshwater biomonitoring for standardisation

10:45 - 10:55  10-min break

10:55 - 11:10  Vasco Elbrecht
Authors: Vasco Elbrecht, Dominik Buchner, Markus Majaneva, Adriana Mordente, Jukka Aroviita, Torbjørn Ekrem, Annette Baattrup-Pedersen, Jón Ólafsson, Richard Johnson, Ann Schartau, Nikolai Friberg, Florian Leese, Meissner Kristian
Technical challenges when scaling up macroinvertebrate DNA metabarcoding

11:10 - 11:25  Anastasija Zaiko
Authors: Anastasija Zaiko, Ulla von Ammon, Jacqui Stuart, Kirsty Smith, Richard Yao, Melissa Welsh, Xavier Pochon, Holly Bowers
Performance and cost-efficiency of eDNA and eRNA capture methodologies: experimental assessment using cultured microalgae

11:25 - 11:40  Mark Louie Lopez
Authors: Mark Louie Lopez, Ya-Ying Lin, Ryuji Machida
Comparing metatranscriptomics, PCR-based methods, and morphology in estimating diversity and composition of zooplankton mock communities

11:40 - 11:55  Luca Mirimin
Authors: Luca Mirimin, Dennis van der Pouw Kraan
Application of microfluidic high-throughput quantitative PCR systems for the monitoring and surveillance of aquatic species

11:55 - 12:10  Sofie Derycke
Authors: Sofie Derycke, Sara Maes, Laure Van den Bulcke, Joran Vanhollebeke, Jan Wittoeck, Hans Hillewaert, Bart Ampe, Annelies Haegeman, Kris Hostens, Annelies De Backer
To blend or not to blend? The role of morphological traits for the detection of marine macrobenthos in bulk DNA and eDNA from the ethanol preservative

12:10 - 12:20  10-min break

12:20 - 12:25  Austen C Thomas
Authors: Austen Thomas, Caren Goldberg
Evolution of a high-throughput environmental DNA sampling platform

12:25 - 12:30  Héloïse Verdier
Authors: Héloïse VERDIER, Lara KONECNY, Christophe MARQUETTE, Tristan LEFEBURE
Toward a simple way to collect eDNA using a 3D-printed passive sampler

12:30 - 12:35  Magnus Jacobsen
Authors: Magnus Jacobsen
Autonomous in situ sampling and analysis of eDNA using an Environmental Sample Processor (ESP)

12:35 - 12:40  Thiago Sanches
Authors: Thiago Sanches
Transect sampling promotes higher detection rates compared to discrete sampling for environmental DNA applications

12:40 - 14:00  Lunch break

March 10 PM

Data Analysis & Storage - WG4 session
Chairs: Alain FRANC, Diego FONTANETO

14:00 - 14:10  Diego Fontaneto, Alain Franc (Session Introduction)
Authors: Diego Fontaneto, Alain Franc
Challenges in data analyses and data storage for metabarcoding

14:10 - 14:35  Benjamin Callahan (Keynote Lecture)
Authors: Benjamin Callahan
Amplicon Sequencing in the Era of Highly-Accurate Long Reads

14:35 - 15:00  Holly Bik (Keynote Lecture)
Authors: Holly Bik
Complexity matters: Evaluating the impact of bioinformatics parameters on eukaryotic MOTU delimitation and taxonomy assignment

15:00 - 15:15  Alain Franc
Authors: Jean-Marc Frigerio, Ester Eckert, Emre Keskin, Frédéric Mahé, Michael Monaghan, Matteo Montagna, Franck Salin, Paul Schmidt Yáñez, Douglas Yu, Diego Fontaneto, Alain Franc
OTU picking on large datasets : comparing methods on a diversity of situations

15:15 - 15:30  Haris Zafeiropoulos
Authors: Haris Zafeiropoulos, Christina Pavloudi, Evangelos Pafilis
PEMA v2: addressing metabarcoding bioinformatics analysis challenges

15:30 - 15:45  Emese Meglécz
Authors:Emese Meglécz, Vincent Dubut, Emmanuel Corse, Aitor González
VTAM: A robust pipeline for validating metabarcoding data using optimized parameters based on internal controls.

15:45 - 15:55  10-min break

15:55 - 16:10  Saskia Oosterbroek
Authors: Saskia Oosterbroek, Karlijn Doorenspleet, Reindert Nijland, Lara Jansen
Decona: From demultiplexing to consensus for Nanopore amplicon data

16:10 - 16:25  Till Macher
Authors: Till-Hendrik Macher, Arne Beermann, Florian Leese
TaxonTableTools - A comprehensive, platform-independent graphical user interface software to explore and visualise DNA metabarcoding data

16:25 - 16:40  Emiliano Pereira-Flores
Authors: Emiliano Pereira-Flores, Albert Barberan, Frank Oliver Glöckner, Antonio Fernandez-Guerra
Mg-Traits pipeline: advancing functional trait-based approaches in metagenomics

16:40 - 16:55  Verena Dully
Authors: Verena Dully, Tom Wilding, Timo Mühlhaus, Thorsten Stoeck
Predicting classifications in marine biomonitoring with supervised machine learning: how much data is required?

16:55 - 17:10  Christopher Hempel
Authors: Christopher Hempel, Julia Harvie, Jose Hleap Lozano, Natalie Wright, Sarah Adamowicz, Dirk Steinke
Comparing total RNA sequencing and metagenomics pipelines for multi-domain taxonomic profiling: implications for ecological assessments

17:10 - 17:20  10-min break

17:20 - 17:25  Ester Maria Eckert
Authors: Ester Maria Eckert
Every fifth published metagenome is not available to science

17:25 - 17:30  Kálmán Tapolczai
Authors: Kálmán Tapolczai, François Keck, Valentin Vasselon, Géza Selmeczy, Maria Kahlert, Agnès Bouchez, Frédéric Rimet, Judit Padisák
Strategies for the bioinformatic treatment of high-throughput sequencing data for diatom studies and bioassessment

17:30 - 17:35  Martin Laporte
Authors: Martin Laporte
Proper environmental DNA Metabarcoding (eMetabarcording) data transformation reveals temporal stability of fish communities in a dendritic river system

17:35 - 17:40  Alfried Vogler
Authors: Alfried Vogler, Md. Mizanur Rahman, Alfred Burian
Metabarcoding to establish freshwater indicators of environmental degradation in the Indo-Burmese biodiversity hotspot

17:40 - 17:45  Aleksandra Jabłońska
Authors: Aleksandra Jablonska, Nicolas Navarro, Remi Laffont, Remi Wattier, Vladimir Pesic, Andrzej Zawal, Jasna Vukic, Michal Grabowski
DNA barcodes combined with geometric morphometry challenge species hypothesis in palaemonid shrimp

17:45 - 18:00  15-min break

18:00 - 20:00  Poster session WG2 & WG5

 

March 11 AM

Implementation Strategy & Legal Issues - WG5 session
Chairs: Patricia MERGEN, Pieter BOETS, Daniel HERING

09:00 - 09:10  Pieter Boets, Daniel Hering, Patricia Mergen (Session Introduction)
Authors: Pieter Boets, Daniel Hering, Patricia Mergen
Implementation and legal issues of DNA-based monitoring (WG 5)

09:10 - 09:35  Florian Altermatt (Keynote Lecture)
Authors: Florian Altermatt
The challenges and opportunities for implementation of eDNA biomonitoring in riverine systemsed

09:35 - 10:00  Kristian Meissner (Keynote Lecture)
Authors: Meissner Kristian
The pathway of molecular methods from research to routine use

10:00 - 10:15  Philippe Blancher
Authors: Philippe Blancher, Estelle Lefrançois, Frederic Rimet, Agnès Bouchez
Strategy for Successful Integration of eDNA-based Methods in Aquatic Monitoring

10:15 - 10:30  Joseph Crain
Authors: Joseph Craine, Nicholas Schulte
Paths toward implementing eDNA-based bioassessments across the United States

10:30 - 10:45  PJ Stephenson
Authors: PJ Stephenson
The use of environmental DNA in monitoring aquatic biodiversity for conservation: a review of challenges and opportunities

10:45 - 10:55  10-min break

10:55 - 11:10  Alexander Weigand
Authors: Alexander Weigand
(e)DNA-based assessments within one of the World's largest river survey programs: summarized insights from the 4th Joint Danube Survey

11:10 - 11:25  Didier Pont
Authors: Didier Pont, Paul Meulenbroeck, Vinzenz Bammer, Tony Dejean, Tibor Eros, Thomas Hein, Pauline Jean, Mirjana Lenhardt, Christoffer Nagel, Ladislav Pekárik, Michael Schabuss, Bernhard Stoeckle, Alexander Weigand, Horst Zornig, Alice Valentini
eDNA metabarcoding survey of fish communities in the Danube and its tributaries

11:25 - 11:40  Nathan Griffiths
Authors: Nathan Griffiths, Rosalind Wright, Bernd Hänfling, Jonathan Bolland, Katerina Drakou, Graham Sellers, Stamatis Zogaris, Marlen Vasquez
The European eel Anguilla anguilla in Cyprus - Investigating the role of freshwaters for eel conservation using integrated monitoring methods

11:40 - 11:55  Jelger Herder
Authors: Jelger Herder, Jan Kranenbarg, Alice Valentini
eDNA metabarcoding fish in relation to the Water Framework Directive indices

11:55 - 12:10  Dalila Destanović
Authors: Dalila Destanović, Lejla Ušanović, Jasna Hanjalić, Lejla Lasić, Belma Kalamujić Stroil
Unheeded biodiversity: perspectives of DNA barcoding of Trichoptera in Bosnia and Herzegovina

12:10 - 12:20  10-min break

12:20 - 12:25  Willie Duncan
Authors: Willie Duncan
Developing DNA Based Methods for Environmental Regulation and Management- UK Insights

12:25 - 12:30  Maarten De Brauwer
Authors: Maarten De Brauwer
A roadmap for integrating eDNA in Australian marine park monitoring

12:30 - 12:35  Kirsten Harper
Authors: Kirsten Harper, Kat Bruce, Cuong Tang, Andrea Ross-Gillespie, Vere Ross-Gillespie, Bastian Egeter
A web-based tool to standardise reporting and interpret results of eDNA qPCR assays

12:35 - 12:40  Lennart Schreiber
Authors: Lennart Schreiber
eDNA reveals widespread presence of marine fish in the Panama Canal

12:40 - 12:45  Thomas Merzi
Authors: Thomas Merzi
Environmental Genomics Applications for Environmental Management Activities in the Oil and Gas Industry - State of the Art Review and Future Research Needs

12:45 - 12:50  Daniela Losacco
Authors: Daniela Losacco, Marina Tumolo, Roberto Mauro, Barbara Casale, Carmine Massarelli, Vito Uricchio, Domenico De Paola
Validation of bacterial markers to discriminate against the source of nitrate contamination: a promising application within the EU Nitrates Directive

12:50 - 14:00  Lunch break

March 11 PM

Biotic Indices & Metrics - WG2 session
Chairs: Maria KAHLERT, Jan PAWLOWSKI

14:00 - 14:10  Maria Kahlert, Jan Pawlowski (Session Introduction)
Authors: Maria Kahlert, Jan Pawlowski
Biotic Indices & Metrics: Achievements of the DNAqua-Net Working Group 2

14:10 - 14:35  Isabelle Domaizon (Session Keynote)
Authors: Isabelle Domaizon
Lake sedimentary DNA to reveal long-term changes in aquatic biodiversity and ecosystem functioning

14:35 - 15:00  Maria Joao Feio (Session Keynote)
Authors: Maria Joao Feio
The use of machine learning predictive models to assess rivers quality with molecular data

15:00 - 15:15  Angel Borja
Authors: Ángel Borja, Iñigo Muxika, Anders Lanzén
Is the ecological status assessment result the same using genomic- and morphology-based benthic biotic indices?

15:15 - 15:30  Alexander Eiler
Authors: Alexander Eiler, Domenico Savio, Laurent Fontaine, Lorenzo Pin
Developing prokaryotic water quality indicators

15:30 - 15:45  Dana Clark
Authors: Dana Clark, Conrad Pilditch, Joanne Ellis, Angel Borja, Javier Atalah, John Pearman, Anastasija Zaiko
eDNA reveals estuarine benthic community response to nutrient enrichment – evidence from an in-situ experiment

15:45 - 15:55  10-min break

15:55 - 16:10  Rosa Trobajo
Authors: Rosa Trobajo, Javier Pérez Burillo, Valentin Vasselon, Frederic Rimet, Agnès Bouchez, David Mann
Species sensitivity analysis as a tool for interpreting diatom metabarcoding for WFD bioassessment

16:10 - 16:25  Anders Lanzén
Authors: Anders Lanzén, Jon Thomassen Hestetun, Andrea Bagi, Thomas Dahlgren
Sediment eDNA Metabarcoding for monitoring impacts from offshore oil extraction

16:25 - 16:40  Bernd Hänfling
Authors: Bernd Hänfling, Nigel Willby, Lori Lawson-Handley, Graham Sellers
101 lakes - lentic fish community monitoring using eDNA metabarcoding

16:40 - 16:55  Larissa Frühe
Authors: Larissa Frühe, Xavier Pochon, Olivier Laroche, Nigel Keeley, Verena Dully, Tristan Cordier, Jan Pawlowski, Thorsten Stoeck
Biogeographic structuring of benthic microbial communities influence the establishment of global bioindicators for coastal aquaculture impact monitoring

16:55 - 17:10  Nadieh de Jonge
Authors: Nadieh de Jonge, Martin Hesselsøe, Jeppe Nielsen
The potential of sediment and fauna microbiomes in water quality assessment

17:10 - 17:20  10-min break

 17:20 - 17:25  Sinziana Rivera
Authors: Sinziana Rivera, Valentin Vasselon, Frederic Rimet, Agnès Bouchez
Aquatic biofilms can act as natural environmental DNA samplers

17:25 - 17:30  Panayiota Pissaridou
Authors: Panayiota Pissaridou, Agnès Bouchez, Marlen Vasquez Hadjilyra, Valentin Vasselon, Andreas Christou, Teofana Chonova, Katerina Drakou, Frederic Rimet, Marco Cantonati, Gerald Dörflinger, Iakovos Tziortzis
Morphotaxonomy- and metabarcoding-based ecological assessment of Cyprus streams’ diatom communities and correlation with environmental and anthropogenic influences

17:30 - 17:35  Marco Cavaliere
Authors: Inés Barrenechea, Marco Cavaliere, Marina Montresor, Carla Bucci, Lorenzo Brocani, Francesca Margiotta, Fabio Francescangeli, Jan Pawlowski, Fabrizio Frontalini
Environmental monitoring of the highly polluted Gulf of Bagnoli (Italy) using foraminiferal metabarcoding and morphology-based assessment

17:35 - 17:40  Luciana Griffero
Authors: Luciana Griffero
Exploring the use of new water quality indicators based on microbial communities

17:40 - 17:45  Jeanine Brantschen
Authors: Jeanine Brantschen, Rosetta Blackman, Florian Altermatt, Jean-Claude Walser
A large-scale ecological assessment of Swiss rivers using environmental DNA for the monitoring of macroinvertebrates

17:45 - 18:05  Florian Leese, Agnès Bouchez, Marlen Vasquez
Ceremony of DNAqua-Net Awards

18:05 - 18:15  Florian Leese, Agnès Bouchez
Closing Adress

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Management Committee of DNAqua-Net

9th March 2021, morning

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DNA Barcode References - WG1 session 

The endless quests for completeness and curation of reference libraries

9th March 2021, afternoon

DNA barcode reference libraries are of immense importance for correct identification of organisms using DNA barcoding and metabarcoding. In this session we welcome contributions that focus on the construction of reference databases and analysis of reference data: from filling the gaps and quality control of existing barcode libraries, to the analyses of species boundaries and use of integrative taxonomy in revision of classifications. 

Chairs: Torbjorn EKREM,  Fedor CIAMPOR

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Field & Lab Protocols - WG3 session

The search for "One (or none) protocol to rule them all!"

10th March 2021, morning

Environmental DNA as a tool in molecular ecology resulted in promising success with detection in higher precision rates and easier field protocols compared to traditional field surveys in aquatic ecosystems. Moreover, after the enhanced progress in the high throughput sequencing technologies, bioassessment of entire communities from related habitats from single or pooled PCR also becomes available without any prior knowledge on the species inhabiting the environment. One of the main challenges in eDNA studies is that the DNA extracted from potentially inhibiting agent rich environmental samples are mostly short and degraded fragments and thus requiring protocol adjustment accordingly. This session will try to highlight outcomes of all possible scenarios and discuss scientific data related to variables such as effects of sampling, filtration, storage conditions, extraction method, primer design/using universal primers, PCR (conventional, quantitative or digital), sequencing (Sanger, pyrosequencing, high throughput).

Chairs: Kat BRUCE, Emre KESKIN

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Data Analysis & Storage - WG4 session

Revealing, comparing and storing biodiversity patterns in massive amplicon datasets

10th March 2021, afternoon

Analyses of biodiversity data from metagenomics face two main issues: one on storing and maintaining large amount of data in online repositories, and the other one on having comparable pipelines to process data. Data processing aims at revealing biodiversity patterns and bridging these patterns with tools and models in statistical ecology. The session will explore the details of the current and expected approaches (i) for storage of raw data and data processing outcomes of massive datasets compliant with FAIR principles, (ii) in the available pipelines to obtain Operational Taxonomic Units, Amplicon Sequence Variants, and other basic units of diversity from massive sequencing; showcasing examples, advantages, caveats, and pitfalls. The main aims are to foster our understanding of biodiversity patterns at different scales by producing reliable and reusable data and pipelines downstream of massive sequencing and thus, enabling cross comparisons.

Chairs: Alain FRANC, Diego FONTANETO

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Implementation Strategy & Legal Issues - WG5 session

Neglected, feared but indispensable:  standardization and tailored communication

11th March 2021, morning

Worldwide, the use of DNA-based monitoring is developing fast and the time has come to focus on standardisation and implementation into routine monitoring. This session welcomes experiences on how to implement these novel techniques into routine water quality or biodiversity monitoring in all kind of aquatic ecosystems. We also are looking for people sharing experiences on how to communicate to stakeholders such as monitoring agencies and regulatory bodies and how to involve them in the decision making. Finally, legal issues and opportunities or threats with regard to the use of DNA-based monitoring will be discussed during this session.     

Chairs: Patricia MERGEN, Pieter BOETS, Daniel HERING

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Biotic Indices & Metrics - WG2 session

New trends in bioassessment of aquatic ecosystems: from organisms to DNA-based metrics

11th March 2021, afternoon

Traditionally, the biological quality of aquatic ecosystems is assessed using selected groups of organisms that can be identified morphologically. Recent advances in high-throughput genomic approaches offered new opportunities to monitor biodiversity and assess ecological status using DNA barcoding and metabarcoding. The DNA-based tools have been used in three different ways: (1) to replace morphological identification of biological quality elements in existing biotic indices, (2) to develop new molecular indices based on morphologically inconspicuous groups of potential environmental indicators, and (3) to predict biotic indices from environmental DNA datasets using machine learning methods. During this session, we will present these different approaches and discuss their advantages and challenges in view of their future application in routine bioassessment.

Chairs: Maria KAHLERT, Jan PAWLOWSKI

See also

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